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KMID : 0368019910140030839
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Journal of Soonchunhyang University
1991 Volume.14 No. 3 p.839 ~ p.845
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Production of the Spore-Crystal Complex by Bacillus thuringiensis var. israelensis with Ground Wood Pulp Mill Waste Liquor.
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Abstract
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Effect of some nutruent on the growth of Bacillus thuringiensis var.israelensis in the froung wood pulp will waste liquor was determined during as attempt to lower the BOD content of the waste liquor and to produce spore-crystal complex by bacteria. The stain applied were commerical Bacillus thuringiensis var.israelensis (Comm. B. t. i) and serotype H-14, Bacillus thuringiensis var.israelensis (Serotype B.t.i), The necessity of the addition of 1.0% peptone 0.05% K_(2)HPO_(4), 0.01% MgSO_(4) 7H_(2)O and 0.02% CaCl_(2) for the best growth of two strains in the waste riquor was ascertained as a result. Comm. B. t. i was at a maximal growth after the 40 hour of incubation but Serotype B. t.i, after the 32 hour of incubation of the strains in the supplemental waste liquor, spore count of Comm. B. t. i and Serotype B. t. i was 8.3 × 10^(9) spores/ml and 6.4 × 10^(9) spores/ml respectively and the BOD content of the waste liquor was lowered by about 70%. Two strains showed 100% of lethal rate, when Culex pipiens larvae were exposed to 10^(8) spores/ml of concentration of spore-crystal complex.
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